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Title: Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres
Authors: Bunker, Shannon;Holeniewska, Joanna;Vijay, Sauparnika;Dahlmann-Noor, Annegret;Khaw, Peng;Ng, Yin-Shan;Shima, David;Foxton, Richard
Keywords: Medicine;Issue 96;Eye;glaucoma;magnetic beads;animal model;intraocular pressure;apoptosis;neuron;degeneration;optic nerve.
Issue Date: 2015
Publisher: MyJove Corporation
Description: Progress in understanding the pathophysiology, and providing novel treatments for glaucoma is dependent on good animal models of the disease. We present here a protocol for elevating intraocular pressure (IOP) in the rat, by injecting magnetic microspheres into the anterior chamber of the eye. The use of magnetic particles allows the user to manipulate the beads into the iridocorneal angle, thus providing a very effective blockade of fluid outflow from the trabecular meshwork. This leads to long-lasting IOP rises, and eventually neuronal death in the ganglion cell layer (GCL) as well as optic nerve pathology, as seen in patients with the disease. This method is simple to perform, as it does not require machinery, specialist surgical skills, or many hours of practice to perfect. Furthermore, the pressure elevations are very robust, and reinjection of the magnetic microspheres is not usually required unlike in some other models using plastic beads. Additionally, we believe this method is suitable for adaptation for the mouse eye.
Other Identifiers: Bunker, Shannon, Joanna Holeniewska, Sauparnika Vijay, Annegret Dahlmann-Noor, Peng Khaw, Yin-Shan Ng, David Shima, and Richard Foxton. 2015. “Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres.” Journal of Visualized Experiments : JoVE (96): 52400. doi:10.3791/52400.
Appears in Collections:HMS Scholarly Articles

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